Does Long-Term Proton Pump Inhibitor Therapy Affect the Health of Gut Microbiota?

Proton pump inhibitors (PPIs) are among the most widely sold medications in the world and are generally considered safe. PPIs are frequently prescribed or taken for long periods without evidence-based indication. 1 However, long-term use of PPIs has not been studied in depth, and several side effects have recently been identified, including an increased risk of enteric infections such as small intestinal bacterial overgrowth and Clostridium difficile infections. 2,3 The increased risk of enteric infections can be caused by the changes in the PPI-user's gut microbiome, which result from the reduced acidity of the stomach and the subsequent survival of more bacteria that are ingested with food and oral mucus. Gut microbiota can resist or promote the colonization of C. difficile and other enteric infections through mechanisms that directly inhibit bacterial growth or enhance the immune system. 4 Through the fecal microbiome analysis of 1,815 individuals spanning three cohorts, PPI use was associated with decreased bacterial richness and profound changes in the gut microbiome. 5 Oral bacteria and potential pathogenic bacteria were increased in the feces of PPI users. In addition, the alterations of fecal microbiota in PPI users were more prominent than individuals who had used antibiotics or other drugs. 5 A recently published, large, healthy twin cohort study identified significant associations between the composition of gut microbiota in feces and PPI use. 6 The best way to confirm that long-term PPI use altered gut microbiota composition is to conduct double-blinded, random-ized, placebo-controlled trials; however, this study may not be feasible due to ethical concerns. Animal models should be investigated to determine the impact of long-term PPI therapy on gut microbiota and to set up preclinical trials to resolve the problem associated with long-term PPI therapy. Until recently, few studies have confirmed the effects of long-term PPI treatment on the gut microbiota in animal models. 7 In this issue of Gut and Liver, the article " Impact of long-term proton pump inhibitor therapy on gut microbiota in F344 rats: pilot study " by Shin et al. 8 investigated compositional changes in gut microbiota due to long-term PPI use in F344 rats treated with lansoprazole for 50 weeks. The gut microbiota in rats' terminal ileum was profiled using 16S rRNA sequencing. Shin et al. 8 found that the profile of gut microbiota in the terminal ileum of F344 rats treated with lansoprazole for 50 weeks was quite different than that …